Effects of Ocular Hypertension in the Visual System of Pigmented Mice

• DOI: 10.1371/journal.pone.0121134
• Publication Date: 2015-03-27T17:20:26Z
• Authors: Francisco J. Vali...; Manuel Salinas-Na...; Manuel Jiménez-López; Luis Alarcón-Mart...; Arturo Ortín-Mart...; José M. Bernal-Garro; Marcelino Avilés-...; Marta Agudo-Barriuso; María P. Villegas...; Manuel Vidal-Sanz
• Keywords: Ganglion cell layer; Axoplasmic transport

To study the effects of ocular hypertension (OHT) on visual system C57BL/6 pigmented mice, limbal and episcleral veins left eye were laser photocoagulated (LP). LP increased intraocular pressure during first five days (d), reaching basal values at 7d. investigate effect OHT retinal ganglion cell (RGC) retrograde axonal transport, hydroxistilbamidine methanesulfonate (OHSt) was applied to both superior colliculi (SCi) retinas dissected 2 or 4 weeks after LP. determine RGC survival, these same immunoreacted against Brn3a (general population) melanopsin (intrinsically photosensitive RGCs, m+RGCs). whether affected non-RGC neurons in layer (GCL), RGCs immunodetected with all GCL nuclei counterstained DAPI a group animals examined post-LP. Innervation SCi 10 days, 8 14 orthogradely transported cholera toxin subunit-B. resulted diffuse sectorial loss OHSt+RGCs (50% 62% weeks) comparable Brn3a+RGCs time intervals. m+RGCs decreased 59% 46% weeks, such diffuse, did not parallel general population more severe superior-temporal retina. In GCL, is selective for does affect other neurons. The retinotectal innervation appeared significantly reduced (55.7%) progress further up (46.6%). Thus, LP-induced results degeneration m+RGCs, as well CTB-labelled terminals.