Integrated RNA-Seq and sRNA-Seq Analysis Identifies Chilling and Freezing Responsive Key Molecular Players and Pathways in Tea Plant (Camellia sinensis)

• DOI: 10.1371/journal.pone.0125031
• Publication Date: 2015-04-22T17:58:31Z
• Authors: Chao Zheng; Lei Zhao; Yu Wang; Jiazhi Shen; Yinfei Zhang; Sisi Jia; Yusheng Li; Zhaotang Ding
• Keywords: RNA-Seq

Tea [Camellia sinensis (L) O. Kuntze, Theaceae] is one of the most popular non-alcoholic beverages worldwide. Cold stress severe abiotic stresses that limit tea plants’ growth, survival and geographical distribution. However, genetic regulatory network signaling pathways involved in cold responses plants remain unearthed. Using RNA-Seq, DGE sRNA-Seq technologies, we performed an integrative analysis miRNA mRNA expression profiling their under chilling (4℃) freezing (-5℃) stress. Differentially expressed (DE) profiles were obtained based on fold change analysis, miRNAs target mRNAs found to show both coherent incoherent relationships network. Furthermore, compared several key (e.g., ‘Photosynthesis’), GO terms ‘response karrikin’) transcriptional factors (TFs, e.g., DREB1b/CBF1) which identified as early and/or response plants. Intriguingly, karrikins, a new group plant growth regulators, β-primeverosidase (BPR), enzyme functionally relevant with formation aroma might play important role Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) further confirmed results from RNA-Seq analysis. This first study simultaneously profile patterns genome-wide scale elucidate molecular mechanisms In addition gaining deeper insight into resistant characteristics plants, provide good case analyse mRNA/miRNA non-model species using next-generation sequencing technology.