Reciprocal Regulation of C-Maf Tyrosine Phosphorylation by Tec and Ptpn22

• DOI: 10.1371/journal.pone.0127617
• Publication Date: 2015-05-20T14:09:12Z
• Authors: Chih-Chun Liu; Chen-Yen Lai; Wei-Feng Yen; Yu-Hsien Lin; Hui-Hsin Chang; Tzong-Shyuan Tai; Yu-Jung Lu; Hsiao-Wei Tsao; I-Cheng Ho; Shi-Chuen Miaw
• Keywords: Dephosphorylation

C-Maf plays an important role in regulating cytokine production TH cells. Its transactivation of IL-4 is optimized by phosphorylation at Tyr21, Tyr92, and Tyr131. However, the molecular mechanism its tyrosine remains unknown. In this study, we demonstrate that Tec kinase family member Tec, but not Rlk or Itk, a c-Maf enhances c-Maf-dependent promoter activity. This effect counteracted Ptpn22, which physically interacts with facilitates dephosphorylation thereby attenuating transcriptional We further show Tyr21/92/131 also critical for recruitment to IL-21 optimal TH17 Thus, manipulating through kinases phosphatases can have significant impact on cell-mediated immune responses.