Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp. phaseoli

• DOI: 10.1371/journal.pone.0127698
• Publication Date: 2015-06-02T00:49:45Z
• Authors: Renfeng Xue; Jing Wu; Zhendong Zhu; Lanfen Wang; Xiaoming Wang; Shumin Wang; Matthew W. Blair
• Keywords: Secondary metabolism; Homology

Fusarium wilt of common bean (Phaseolus vulgaris L.), caused by oxysporum Schlechtend.:Fr. f.sp. phaseoli (Fop), is one the most important diseases beans worldwide. Few natural sources resistance to Fop exist and provide only moderate or partial levels protection. Despite economic importance disease across multiple crops, a few induced genes have been analyzed in legumes. Therefore, our goal was identify transcriptionally regulated during an incompatible interaction between pathogen using cDNA amplified fragment length polymorphism (cDNA-AFLP) technique. We generated total 8,730 transcript-derived fragments (TDFs) with 768 primer pairs based on comparison moderately resistant susceptible genotype. In total, 423 TDFs (4.9%) displayed altered expression patterns after inoculation inoculum. obtained full amplicon sequences for 122 selected TDFs, which 98 were identified as annotated known different functional categories their putative functions, 10 predicted but non-annotated 14 not homologous any genes. The encoding function classified related metabolism (22), signal transduction (21), protein synthesis processing (20), development cytoskeletal organization (12), transport proteins (7), gene RNA (4), redox reactions defense stress responses (3), energy hormone (2). Based analyses homology, 19 from chosen analysis quantitative RT-PCR. found be here implicated at various steps infection will allow better understanding mechanisms similar pathogens. differential response discovered could also used molecular markers association mapping QTL analysis.