Modulation of Human Valve Interstitial Cell Phenotype and Function Using a Fibroblast Growth Factor 2 Formulation

• DOI: 10.1371/journal.pone.0127844
• Publication Date: 2015-06-04T18:00:55Z
• Authors: Najma Latif; Alfred Quillon; Padmini Sarathcha...; Ann McCormack; Alec Lozanoski; Magdi H. Yacoub; Adrian H. Chester
• Keywords: Modulation (music)

Valve interstitial cells (VICs) are fibroblastic in nature however culture it is widely accepted that they differentiate into a myofibroblastic phenotype. This study assessed fibroblast media formulation for its ability to maintain the phenotype and function of VICs as intact healthy valve. Normal human were cultured separately standard DMEM consisting FGF2 (10ng/ml), insulin (50ng/ml) 2% FCS at least week. Cell morphology, aspect ratio, size, levels distribution protein expression, proliferation, cell cycle, contraction migration assessed. Some some valve endothelial expressed tissue this expression was increased calcified valves. exhibited large, spread whereas smaller, elongated spindly. Aspect ratio size both significantly higher (p<0.01). The level α-SMA reduced day 2 after isolation (p<0.01) α-SMA, SM22 EDA-fibronectin days 7 12 post-isolation Expression cytoskeletal proteins, bone marker proteins extracellular matrix media. Proliferation weeks 1 (p<0.05) Collagen gel (p<0.05). found have fewer smaller focal adhesions with supermature (p<0.001). Ultrastructurally, resembled native from demonstrated slower migratory wounding 72 hours Treatment has dedifferentiate back phenotypic functional characteristics ascribed methodology fundamental normal biology, pathology field engineering.