Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family

• DOI: 10.1371/journal.pone.0128025
• Publication Date: 2015-06-09T13:49:50Z
• Authors: Runyararo M. Hove; Mark Ziemann; Mrinal Bhave
• Keywords: Hordeum; Water Transport; Biotic stress

Aquaporins (AQPs) are major intrinsic proteins (MIPs) that mediate bidirectional flux of water and other substrates across cell membranes, play critical roles in plant-water relations, dehydration stress responses crop productivity. However, limited data available as yet on the contributions these to physiology barley (Hordeum vulgare). The present work reports identification expression analysis MIP family. A comprehensive search publicly leaf mRNA-seq data, draft genome GenBank transcripts sixteen new annotations together revealed family is comprised at least forty AQPs. Alternative splicing events were likely two plasma membrane protein (PIP) Analyses AQP signature sequences specificity determining positions indicated a potential several putative isoforms transport non-aqua including physiological important substrates, respond abiotic stresses. Analysis our identified notable differential HvPIP1;2 HvTIP4;1 under salt stress. gene resources also confirmed isoform-specific different tissues and/or response salinity, well some potentially inter-cultivar differences. systematic most, if not all, genes, their sequences, patterns tissues, functions, strong framework for selection development tolerant varieties. In addition, would be highly valuable genetic studies evolutionarily closely related wheat (Triticum aestivum L.).