Gene Expression Profiling in Peripheral Blood Cells and Synovial Membranes of Patients with Psoriatic Arthritis

• DOI: 10.1371/journal.pone.0128262
• Publication Date: 2015-06-18T18:12:54Z
• Authors: Marzia Dolcino; Andrea Ottria; Alessandro Barbieri; Giuseppe Patuzzo; Elisa Tinazzi; Giuseppe Argentino; Ruggero Beri; Claudio Lunardi; Antonio Puccetti
• Keywords: Pathogenesis

Psoriatic arthritis (PsA) is an inflammatory whose pathogenesis poorly understood; it characterized by bone erosions and new formation. The diagnosis of PsA mainly clinical diagnostic biomarkers are not yet available. aim this work was to clarify some aspects the disease identify specific gene signatures in paired peripheral blood cells (PBC) synovial biopsies patients with PsA. Moreover, we tried that can be used practice.PBC 10 were study expression using Affymetrix arrays. values validated Q-PCR, FACS analysis detection soluble mediators.Synovial showed a modulation approximately 200 genes when compared healthy donors. Among differentially expressed observed upregulation Th17 related type I interferon (IFN) inducible genes. confirmed polarization. trascriptome shows clusters (bone remodeling, angiogenesis inflammation) involved Interestingly 90 modulated both compartments (PBC synovium) suggesting signature pathways PBC mirror those inflamed synovium. Finally osteoactivin upregulared finding high levels sera but other arthritides.We describe first trancriptome tissue This strengthens hypothesis autoimmune origin since coactivity IFN typical autoimmunity. these findings have allowed identification possible biomarker, osteoactivin, easily detectable serum.