Head and Neck Veins of the Mouse. A Magnetic Resonance, Micro Computed Tomography and High Frequency Color Doppler Ultrasound Study

• DOI: 10.1371/journal.pone.0129912
• Publication Date: 2015-06-11T21:19:32Z
• Authors: Marcello Mancini; Adelaide Greco; Enrico Tedeschi; Giuseppe Palma; Monica Ragucci; Maria Grazia Bruz...; Anna Rita Daniela...; Enza Torino; Alessandro Scotti; Ileana Zucca; Marco Salvatore
• Keywords: Male; Science; 610; High frequency ultrasound; Color Doppler.; Mice; 03 medical and health sciences; Computed Tomography; 0302 clinical medicine; 616; Animals; Ultrasonics; ex-vivo Microcomputed tomography; Q; cerebral circulation; R; veins; X-Ray Microtomography; Cerebral Veins; Echocardiography, Doppler, Color; Mice, Inbred C57BL; High frequency ultrasound, Magnetic Resonance Angiography, ex-vivo Microcomputed tomography; Medicine; Jugular Veins; Head; Magnetic Resonance Angiography; Neck; Research Article

To characterize the anatomy of the venous outflow of the mouse brain using different imaging techniques. Ten C57/black male mice (age range: 7-8 weeks) were imaged with high-frequency Ultrasound, Magnetic Resonance Angiography and ex-vivo Microcomputed tomography of the head and neck. Under general anesthesia, Ultrasound of neck veins was performed with a 20 MHz transducer; head and neck Magnetic Resonance Angiography data were collected on 9.4 T or 7 T scanners, and ex-vivo Microcomputed tomography angiography was obtained by filling the vessels with a radiopaque inert silicone rubber compound. All procedures were approved by the local ethical committee. The dorsal intracranial venous system is quite similar in mice and humans. Instead, the mouse Internal Jugular Veins are tiny vessels receiving the sigmoid sinuses and tributaries from cerebellum, occipital lobe and midbrain, while the majority of the cerebral blood, i.e. from the olfactory bulbs and fronto-parietal lobes, is apparently drained through skull base connections into the External Jugular Vein. Three main intra-extracranial anastomoses, absent in humans, are: 1) the petrosquamous sinus, draining into the posterior facial vein, 2) the veins of the olfactory bulb, draining into the superficial temporal vein through a foramen of the frontal bone 3) the cavernous sinus, draining in the External Jugular Vein through a foramen of the sphenoid bone. The anatomical structure of the mouse cranial venous outflow as depicted by Ultrasound, Microcomputed tomography and Magnetic Resonance Angiography is different from humans, with multiple connections between intra- and extra-cranial veins.