Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs

• DOI: 10.1371/journal.pone.0132420
• Publication Date: 2015-07-10T18:20:08Z
• Authors: Jihan Xia; Yuanyuan Zhang; Leilei Xin; Siyuan Kong; Yaoxing Chen; Shulin Yang; Kui Li
• Keywords: Male; 0301 basic medicine; Time Factors; Swine; Science; Cardiomegaly; Diet, High-Fat; Real-Time Polymerase Chain Reaction; 03 medical and health sciences; Animals; Cluster Analysis; Myocytes, Cardiac; Obesity; Dyslipidemias; Oligonucleotide Array Sequence Analysis; 2. Zero hunger; Staining and Labeling; Gene Expression Profiling; Myocardium; Q; R; Reproducibility of Results; 3. Good health; Gene Ontology; Gene Expression Regulation; Medicine; Swine, Miniature; Female; Research Article

A long-term high-energy diet affects human health and leads to obesity metabolic syndrome in addition cardiac steatosis hypertrophy. Ectopic fat accumulation the heart has been demonstrated be a risk factor for disorders, but molecular mechanism of disease remains largely unknown. Bama miniature pigs were fed high-fat, high-sucrose (HFHSD) 23 months. These developed symptoms showed hypertrophy with greatly increased body weight (2.73-fold, P<0.01), insulin level (4.60-fold, (1.82-fold, P<0.05) volume (1.60-fold, compared control pigs. To understand mechanisms hypertrophy, nine pig cRNA samples hybridized porcine GeneChips. Microarray analyses revealed that 1,022 genes significantly differentially expressed (P<0.05, ≥1.5-fold change), including 591 up-regulated 431 down-regulated HFHSD group relative group. KEGG analysis indicated observed disorder involved signal transduction-related MAPK, cytokine, PPAR signaling pathways, energy metabolism-related fatty acid oxidative phosphorylation function signaling-related focal adhesion, axon guidance, hypertrophic cardiomyopathy actin cytoskeleton inflammation apoptosis others. Quantitative RT-PCR assays identified several important heart-related genes, STAT3, ACSL4, ATF4, FADD, PPP3CA, CD74, SLA-8, VCL, ACTN2 FGFR1, which may targets further research. This study shows long-term, induces obesity, steatosis, provides insights into facilitate