Degradation of Biofumigant Isothiocyanates and Allyl Glucosinolate in Soil and Their Effects on the Microbial Community Composition

• DOI: 10.1371/journal.pone.0132931
• Publication Date: 2015-07-17T18:00:50Z
• Authors: Franziska S. Hans...; Bunlong Yim; Traud Winkelmann; Kornelia Smalla; Monika Schreiner
• Keywords: Myrosinase; Allyl isothiocyanate; Brassicaceae; Degradation; White mustard

Brassicales species rich in glucosinolates are used for biofumigation, a process based on releasing enzymatically toxic isothiocyanates into the soil. These hydrolysis products volatile and often reactive compounds. Moreover, can be degraded also without presence of hydrolytic enzyme myrosinase which might contribute to bioactive effects. Thus, present study stability Brassicaceae plant-derived pure was studied using three different soils (model biofumigation). In addition, degradation 2-propenyl glucosinolate investigated with special regard formation breakdown products. Finally, influence bacterial community composition evaluated denaturing gradient gel electrophoresis 16S rRNA gene amplified from total DNA. The model biofumigation revealed that structure had significant impact their soil but not type. Following soils, nitrile as well isothiocyanate main products, depending Furthermore, shown both chemically biologically mediated autoclaving reduced degradation. major product chemical its increased iron content Additionally, significantly affected by adding glucosinolate, effect being more pronounced than treatments added glucosinolate. Therefore, have greater