Amniotic Membrane Modifies the Genetic Program Induced by TGFß, Stimulating Keratinocyte Proliferation and Migration in Chronic Wounds

• DOI: 10.1371/journal.pone.0135324
• Publication Date: 2015-08-18T13:53:17Z
• Authors: Antonia Alcaraz; Anna Mrowiec; Carmen Luisa Insa...; Ángel Bernabé-García; Eva María García-...; María Concepción ...; Asunción Monfort; Ander Izeta; José María Moraleda; Gregorio Castellanos; Francisco José Ni...
• Keywords: Epidermis (zoology)

Background Post-traumatic large-surface or deep wounds often cannot progress to reepithelialisation because they become irresponsive in the inflammatory stage, so intervention is necessary provide final sealing epidermis. Previously we have shown that Amniotic Membrane (AM) induced a robust epithelialisation traumatic wounds. Methods and Findings To better understand this phenomenon, used keratinocytes investigate effect of AM on chronic Using keratinocytes, saw treatment able exert an attenuating upon Smad2 Smad3 TGFß-induced phosphorylation while triggering activation several MAPK signalling pathways, including ERK JNK1, 2. This also has consequence for regulation cell cycle control key players CDK1A (p21) CDK2B (p15). The study wider set TGFß regulated genes showed was not wide but very concrete some genes. exerted powerful arrest; presence however prevented arrest. Moreover, migration response correlates well with expression c-Jun protein at border healing assay. Consistently, human wound border. Conclusions modulation responses favours proliferation together AM-induced keratinocyte perfect match allows move from their non-healing state into epithelialization. Our results may explain why application promote epithelialisation.