Macrophage Bactericidal Activities against Staphylococcus aureus Are Enhanced In Vivo by Selenium Supplementation in a Dose-Dependent Manner

• DOI: 10.1371/journal.pone.0135515
• Publication Date: 2015-09-04T17:51:17Z
• Authors: Mourad Aribi; Warda Meziane; Salim Habi; Yasser Boulatika; Hélène Marchandin; Jean-Luc Aymeric
• Keywords: Selenium deficiency

Background Dietary selenium is of fundamental importance to maintain optimal immune function and enhance immunity during infection. To this end, we examined the effect on macrophage bactericidal activities against Staphylococcus aureus. Methods Assays were performed in golden Syrian hamsters peritoneal macrophages cultured with S. aureus different concentrations selenium. Results Infected selenium-supplemented animals have significantly decreased levels serum nitric oxide (NO) production when compared infected but non-selenium-supplemented at day 7 post-infection (p < 0.05). A low dose 5 ng/mL induced a significant decrease NO production, increase hydrogen peroxide (H2O2) (respectively, p = 0.009, 0.001). The H2O2 increased increasing selenium; activity reached 20 ng/mL. concentration bacterial arginase activity. growth 0.0001) phagocytic activity, production/arginase balance killing (for all comparisons, Conclusions Selenium acts dose-dependent manner activation, phagocytosis suggesting that inadequate doses may cause loss supplementation could vivo control response