Molecular and Phenotypic Characterization of Staphylococcus epidermidis Isolates from Healthy Conjunctiva and a Comparative Analysis with Isolates from Ocular Infection

• DOI: 10.1371/journal.pone.0135964
• Publication Date: 2015-08-14T18:14:34Z
• Authors: Luis A. Flores-Páez; Juan C. Zenteno; María D. Alcántar...; Carlos F. Vargas-...; Sandra Rodríguez-...; Mario E. Cancino-...; Janet Jan-Roblero; Juan C. Cancino-Diaz
• Keywords: Multilocus sequence typing; SCCmec

Staphylococcus epidermidis is a common commensal of healthy conjunctiva and it can cause endophthalmitis, however its presence in conjunctivitis, keratitis blepharitis unknown. Molecular genotyping S. from could provide information about the origin strains that infect eye. In this paper two collections were used: one ocular infection (n = 62), another 45). All isolates genotyped by pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec), detection genes icaA, icaD, IS256 polymorphism type agr locus. The phenotypic data included biofilm production antibiotic resistance. results displayed 61 PFGE types 107 they highly discriminatory. MLST analysis generated total 25 STs, which 11 STs distributed among lineage ST2 was most frequent (48.4%), while 14 present ST5 abundant (24.4%). By means principal coordinates (PCoA) discriminant (DA) found had as markers III or II, SCCmec V I, mecA gene, resistance to tobramycin, positive biofilm, IS256+. contrast isolates, discriminating chloramphenicol, ciprofloxacin, gatifloxacin oxacillin. biomarkers examined 3 [two with ST9] (3/45, 6.66%) similar genotypic characteristics therefore small population an infection. These suggest do not, almost all cases, eye due their large difference isolates.