The Oral Microbiome of Denture Wearers Is Influenced by Levels of Natural Dentition

• DOI: 10.1371/journal.pone.0137717
• Publication Date: 2015-09-14T18:43:32Z
• Authors: Lindsay E. O’Donnell; Douglas Robertson; Christopher J. Nile; Laura J. Cross; Marcello Riggio; Andrea Sherriff; David Bradshaw; Margaret Lambert; Jennifer Malcolm; Mark J. Buijs; Egija Zaura; Wim Crielaard; Bernd W. Brandt; Gordon Ramage
• Keywords: Oral Microbiome

Objectives The composition of dental plaque has been well defined, whereas currently there is limited understanding the denture and how it directly influences related stomatitis (DS). aims this study were to compare microbiomes wearers, understand implications these towards inter-kingdom host-pathogen interactions within oral cavity. Methods Swab samples obtained from 123 participants wearing either a complete or partial denture; bacterial each sample was determined using bar-coded illumina MiSeq sequencing hypervariable V4 region 16S rDNA. Sequencing data processing undertaken QIIME, clustered in Operational Taxonomic Units (OTUs) assigned taxonomy. dentures sonicated remove microbial flora residing on prosthesis, sonicate then cultured diagnostic colorex Candida media. Samples unstimulated saliva antimicrobial peptides (AMP) levels measured by ELISA. Results We have shown that plaques are significantly distinct both diversity microbiome wearer variable influenced location mouth. Dentures mucosa predominantly made up Bacilli Actinobacteria. Moreover, presence natural teeth significant impact overall composition, when compared fully edentulous. Furthermore, increasing spp. positively correlate with Lactobacillus AMPs quantified, though showed no specific correlations. Conclusions This first provide detailed wearers provided evidence DS development more complex than simply candidal infection. Both fungal kingdoms clearly play role defining progression DS, we unable show defined for AMPs.