Molecular Characterization of LjABCG1, an ATP-Binding Cassette Protein in Lotus japonicus

• DOI: 10.1371/journal.pone.0139127
• Publication Date: 2015-09-29T14:04:37Z
• Authors: Akifumi Sugiyama; Shoju Fukuda; Kojiro Takanashi; Miki Yoshioka; Hirofumi Yoshioka; Yoshihiro Narusaka; Mari Narusaka; Mikiko Kojima; Hitoshi Sakakibara; Nobukazu Shitan; Shusei Sato; Satoshi Tabata; Masayoshi Kawaguchi; Kazufumi Yazaki
• Keywords: Lotus japonicus

LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins model legume, Lotus japonicus, was reported as gene highly expressed during the early stages nodulation, but have not been characterized in detail. In this study we showed that induction LjABCG1 expression remarkable by methyl jasmonate treatment, and reporter experiments indicated strongly nodule parenchyma cell layers adjacent to root vascular tissue toward nodule. suggested be localized at plasma membrane based on fractionation microsomal membranes well separation via aqueous two-phase partitioning. The physiological functions symbiosis pathogenesis were analyzed homologous heterologous systems. knock-down L. japonicus plants did show clear phenotypic differences formation, defense against Pseudomonas syringae, either. contrast, when Arabidopsis pdr8-1 mutant, penetration frequency Phytophthora infestans, potato late blight pathogen, significantly reduced LjABCG1/pdr8-1 than plants. This finding least partially, complemented phenotype pdr8 Arabidopsis, suggesting multiple roles protein plant-microbe interactions.