Genotypic and Phenotypic Heterogeneity in Alicyclobacillus acidoterrestris: A Contribution to Species Characterization

• DOI: 10.1371/journal.pone.0141228
• Publication Date: 2015-10-20T18:20:48Z
• Authors: Antonio Bevilacqua; Monica Mischitelli; Valeria Pietropaolo; Emanuela Ciuffreda; Milena Sinigaglia; Maria Rosaria Corbo
• Keywords: Soil microbiology; Axenic

Alicyclobacillus acidoterrestris is the main cause of most spoilage problems in fruit juices and acidic products. Since soil borne species often contaminate do not need strict extreme requirements for survival, it a great concern to investigate whether how could evolve from their ecological niches microbial community new environments as juices. In this study, 23 isolates thermo-acidophilic, spore-forming bacteria were characterized by cultural molecular methods. addition, 2 strains isolated incident pear juice typed. Strains phenotyping showed that they be grouped into 3 different clusters, some identical or quite similar patterns. Analyzing pH temperature ranges growth, majority able grow at values described many Alicyclobacillus. Qualitative utilization lysine, arginine indole production tryptophan revealed, first time, deamination lysine decarboxylation arginine. Resistance 5% NaCl well ability hydrolyze starch gelatin, nitrate reduction, catalase oxidase activities confirmed literature evidences. Examining 16S rRNA, divided three blocks represented effectively are moving other possible growing source parameters strongly influence bacterial survival. RAPD PCR technique evidenced variability banding patterns and, although was obtain genotypically well-distinguished groups, feasible appreciate genetic similarity between strains. conclusion, investigation entails combination metagenomic classic culture-dependent approaches expand our knowledge about look subspecies.