Clinico-Pathological Association of Delineated miRNAs in Uveal Melanoma with Monosomy 3/Disomy 3 Chromosomal Aberrations

• DOI: 10.1371/journal.pone.0146128
• Publication Date: 2016-01-26T18:41:10Z
• Authors: Nalini Venkatesan; Jagat Kanwar; Perinkulam Ravi D...; Vikas Khetan; Tamsyn M. Crowley; Rajeswari Raguraman; Ganesan Sugneswari; Pukhraj Rishi; Viswanathan Natar...; Jyotirmay Biswas; Subramanian Krish...
• Keywords: Monosomy

To correlate the differentially expressed miRNAs with clinico-pathological features in uveal melanoma (UM) tumors harbouring chromosomal 3 aberrations among South Asian Indian cohort.Based on aberration, UM (n = 86) were grouped into monosomy (M3; n 51) and disomy (D3; 35) by chromogenic in-situ hybridisation (CISH). The recorded. miRNA profiling was performed formalin fixed paraffin embedded (FFPE) samples 6) using Agilent, Human microarray, 8x15KV3 arrays. association between studied univariate multivariate analysis. miRNA-gene targets predicted Target-scan MiRanda database. Significantly dys-regulated validated FFPE mRNAs frozen 10) qRT-PCR. Metastasis free-survival expressions analysed Kaplen-Meier analysis tissues 52).Unsupervised revealed 585 while supervised demonstrated 82 (FDR; Q 0.0). Differential expression of 8 miRNAs: miR-214, miR-149*, miR-143, miR-146b, miR-199a, let7b, miR-1238 miR-134 studied. Gene target prediction SMAD4, WISP1, HIPK1, HDAC8 C-KIT as post-transcriptional regulators miR-134. Five (miR-214, miR146b, miR-199a miR-134) found to be M3/ D3 tumors. In patients liver metastasis, miR-149* strongly correlated.UM can stratified from sections. predicting metastasis survival have been identified. Mechanistic linkage de-regulated miRNA/mRNA provide new insights their role progression aggressiveness.