Co-Circulation of Canine Coronavirus I and IIa/b with High Prevalence and Genetic Diversity in Heilongjiang Province, Northeast China

• DOI: 10.1371/journal.pone.0146975
• Publication Date: 2016-01-15T19:03:15Z
• Authors: Xinyu Wang; Chunqiu Li; Donghua Guo; Xinyu Wang; Shan Wei; Yufei Geng; Enyu Wang; Zhihui Wang; Xiwen Zhao; Mingjun Su; Qiujin Liu; Siyao Zhang; Li Feng; Dongbo Sun
• Keywords: 2019-20 coronavirus outbreak; Circulation (fluid dynamics)

To trace the evolution of canine coronavirus (CCoV), 201 stool samples from diarrheic dogs in northeast China were subjected to reverse transcription-polymerase chain reactions (RT-PCRs) targeting partial M and S genes CCoV, followed by an epidemiological analysis. gene RT-PCRs showed that 28.36% (57/201) positive for CCoV; 57 samples, CCoV-I CCoV-II accounted 15.79% (9/57) 84.21% (48/57), respectively. A sequence comparison revealed nucleotide homologies 88.4%-100% among CCoV strains, 88.7%-96.2% identity between strains Chinese reference strain HF3. The exhibited genetic diversity when compared with other countries. high co-infection rates kobuvirus (CaKV) (33.33%) parvovirus-2 (CPV-2) (31.58%). prevalence differed significantly immunization status, regions, seasons, ages. Moreover, 28 amplified CCoV-positive including 26 CCoV-IIa one CCoV-IIb strain, strain. 86.3%-100% 89.6%-92.2% V1. Our data provide evidence CCoV-I, CCoV-IIa, co-circulate diarrhoetic China, CaKV CPV-2 observed, diversity.