Fabrication of a Selective and Sensitive Sensor Based on Molecularly Imprinted Polymer/Acetylene Black for the Determination of Azithromycin in Pharmaceuticals and Biological Samples

• DOI: 10.1371/journal.pone.0147002
• Publication Date: 2016-01-28T18:43:23Z
• Authors: Tingting Zhou; Yun Tao; Hua Jin; Bin Song; Tao Jing; Dan Luo; Yusun Zhou; Yikai Zhou; Yong-Ill Lee; Surong Mei
• Keywords: Molecularly imprinted polymer; Differential pulse voltammetry; NIP; Carbon paste electrode

A new selective and sensitive sensor based on molecularly imprinted polymer/acetylene black (MIP/AB) was developed for the determination of azithromycin (AZM) in pharmaceuticals biological samples. The MIP AZM synthesized by precipitation polymerization. AB were then respectively introduced as elements preparation MIP/AB-modified carbon paste (MIP/ABP) electrode. performance obtained estimated cyclic voltammetry (CV) differential pulse (DPV) techniques. Compared with non-molecularly polymer (NIP) electrodes, NIP/ABP MIP-modified MIP/ABP electrode exhibited excellent current response toward AZM. prepared also good selectivity comparison structurally similar compounds. effect composition, extraction parameters, electrolyte conditions investigated. Under optimized conditions, showed two dynamic linear ranges 1.0 × 10−7 mol L−1 to 2.0 10−6 10−5 L−1, a limit detection 1.1 10−8 L−1. These predominant properties ensured that exhibits reliability detecting fluids without assistance any separation results validated high-performance liquid chromatography–tandem mass spectrometry method.