The FgNot3 Subunit of the Ccr4-Not Complex Regulates Vegetative Growth, Sporulation, and Virulence in Fusarium graminearum

• DOI: 10.1371/journal.pone.0147481
• Publication Date: 2016-01-22T20:57:08Z
• Authors: Duc-Cuong Bui; Hokyoung Son; Ji Young Shin; Jin-Cheol Kim; Hun Kim; Gyung Ja Choi; Yin-Won Lee
• Keywords: Spore germination; Conidiation; Fungal protein

The Ccr4-Not complex is evolutionarily conserved and important for multiple cellular functions in eukaryotic cells. In this study, the biological roles of FgNot3 subunit were investigated plant pathogenic fungus Fusarium graminearum. Deletion FgNOT3 resulted retarded vegetative growth, spore germination, swollen hyphae, hyper-branching. ΔFgnot3 mutants also showed impaired sexual asexual sporulation, decreased virulence, reduced expression genes related to conidiogenesis. Fgnot3 deletion sensitive thermal stress, whereas NOT3 orthologs other model eukaryotes are known be required cell wall integrity. We found that as a negative regulator production secondary metabolites, including trichothecenes zearalenone. Further functional characterization components Not module demonstrated conserved. Each primarily within context might have distinct outside F. This first study functionally characterize filamentous fungi provides novel insights into signal transduction pathways fungal development.