Matricellular Protein Periostin Mediates Intestinal Inflammation through the Activation of Nuclear Factor κB Signaling

• DOI: 10.1371/journal.pone.0149652
• Publication Date: 2016-02-18T16:29:20Z
• Authors: Seong-Joon Koh; Younjeong Choi; Byeong Gwan Kim; Kook Lae Lee; Dae Woo Kim; Jung Ho Kim; Ji Won Kim; Joo Sung Kim
• Keywords: Male; Colon; Science; Cell Line; 03 medical and health sciences; Animals; Humans; Gene Silencing; Inflammation; 0303 health sciences; Q; Interleukin-8; R; NF-kappa B; Integrin alphaV; Antibodies, Neutralizing; 3. Good health; Intestines; Mice, Inbred C57BL; Disease Models, Animal; Enterocytes; Acute Disease; Medicine; Cytokines; Colitis, Ulcerative; Cell Adhesion Molecules; Research Article; Protein Binding

Periostin is a matricellular protein that interacts with various integrin molecules on the cell surface. Although periostin is expressed in inflamed colonic mucosa, its role in the regulation of intestinal inflammation remains unclear. We investigated the role of periostin in intestinal inflammation using Postn-deficient (Postn-/-) mice. Intestinal epithelial cells (IECs) were transfected by Postn small interfering RNAs. Periostin expression was determined in colon tissue samples from ulcerative colitis (UC) patients. Oral administration of dextran sulfate sodium (DSS) or rectal administration of trinitrobenzene sulfonic acid, induced severe colitis in wild-type mice, but not in Postn-/- mice. Administration of recombinant periostin induced colitis in Postn-/- mice. The periostin neutralizing-antibody ameliorated the severity of colitis in DSS-treated wild-type mice. Silencing of Postn inhibited inteleukin (IL)-8 mRNA expression and NF-κB DNA-binding activity in IECs. Tumor necrosis factor (TNF)-α upregulated mRNA expression of Postn in IECs, and recombinant periostin strongly enhanced IL-8 expression in combination with TNF-α, which was suppressed by an antibody against integrin αv (CD51). Periostin and CD51 were expressed at significantly higher levels in UC patients than in controls. Periostin mediates intestinal inflammation through the activation of NF-κB signaling, which suggests that periostin is a potential therapeutic target for inflammatory bowel disease.