Antitumor and Antimetastatic Effect of Small Immunostimulatory RNA against B16 Melanoma in Mice

• DOI: 10.1371/journal.pone.0150751
• Publication Date: 2016-03-16T20:15:40Z
• Authors: Tatyana O. Kabilova; Aleksandra V. Sen...; Valeriy P. Nikolin; Nelly A. Popova; Marina A. Zenkova; Valentin V. Vlassov; Elena L. Chernolo...
• Keywords: Splenocyte

Small interfering RNAs, depending on their structure, delivery system and sequence, can stimulate innate adaptive immunity. The aim of this study was to investigate the antitumor antimetastatic effects immunostimulatory 19-bp dsRNA with 3'- trinucleotide overhangs (isRNA) melanoma B16 in C57Bl/6 mice. Recently developed novel cationic liposomes 2X3-DOPE were used for vivo isRNA. Administration isRNA/2X3-DOPE complexes significantly inhibits tumor growth metastasis. Histopathological analysis spleen cross sections showed hyperplasia lymphoid white pulp formation large germinal centers after administration, indicating activation immune system. treatment melanoma-bearing mice decreases destructive changes liver parenchyma. Thus, isRNA displays pronounced immunostimulatory, properties against may be considered a potential agent immunotherapy melanoma.