Candidate Soluble Immune Mediators in Young Women with High-Risk Human Papillomavirus Infection: High Expression of Chemokines Promoting Angiogenesis and Cell Proliferation

• DOI: 10.1371/journal.pone.0151851
• Publication Date: 2016-03-18T23:02:59Z
• Authors: Nunzia Zanotta; Maria Lina Tornes...; Clorinda Annunziata; Giovanni Stellato; Franco Maria Buon...; Manola Comar
• Keywords: CXCL9; CCL7

Background The causal interpretation of cervical immune response to Human Papillomavirus (HPV) infection is complex and poorly characterized mainly due the delicate balance that exists between viral infection, increase inflammatory cytokines host risk factors. This study aims explore significance mediators associated cell survival, angiogenesis interaction with response, in predicting develop HPV-related intraepithelial lesions. Methods A panel 48 growth factors were explored a selected cohort 168 immunocompetent women including 88 diagnosed low (LSIL) or high (HSIL) squamous lesions cervix 80 normal cytology (NIL). HPV genotyping was performed by Linear Array test soluble concentration molecules analyzed using Bio-Plex platform. Results prevalence single HR-HPV 30% NIL 100% LSIL HSIL women. expression 13 cytokines, interleukins IL-6, IL-3, IL-12p40, IL-12p70, IL-16, IL-18, LIF, chemokines CCL7 (MCP-3), CXCL9 (MIG), CXCL12 (SDF-1α) tropic VEGF, G-CSF, M-CSF significantly presence levels being higher precancerous compared negative Only factor GM-CSF positively cytological abnormalities. Conclusions ability escape from innate recognition orchestrate production specific factors, involved early cell-proliferating phase damage, documented before development