A Proteomics Approach to Identify New Putative Cardiac Intercalated Disk Proteins

• DOI: 10.1371/journal.pone.0152231
• Publication Date: 2016-05-05T17:55:37Z
• Authors: Siddarth Soni; Antonia J. A. Raa...; Linsey M. Raaijma...; J. Mirjam A. Damen; Leonie van Stuijv...; Marc A. Vos; Albert J. R. Heck; Toon A. B. van Veen; Arjen Scholten
• Keywords: Proteome; Proteogenomics; Intercalated disc

Aims Synchronous beating of the heart is dependent on efficient functioning cardiac intercalated disk (ID). The ID composed a complex protein network enabling electrical continuity and chemical communication between individual cardiomyocytes. Recently, several different studies have shed light increasingly prevalent diseases involving ID. Insufficient knowledge its composition makes it difficult to study these disease mechanisms in more detail therefore here we aim expand proteome. Here, using combination general membrane enrichment, in-depth quantitative proteomics an intracellular location driven bioinformatics approach, discover new putative proteins rat ventricular tissue. Methods Results General isolation, enriched amongst others also with as based presence established markers connexin-43 n-cadherin, was performed centrifugation. By mass spectrometry, quantitatively evaluated level 3455 fraction (EMF) counterpart, soluble cytoplasmic fraction. These data were stringently filtered generate final set 97 enriched, proteins. included Cx43 but many interesting novel candidates. We selected 4 candidates (Flotillin-2 (FLOT2), Nexilin (NEXN), Popeye-domain-containg-protein 2 (POPDC2) thioredoxin-related-transmembrane-protein (TMX2)) confirmed their co-localization n-cadherin human cryo-sections, isolated dog Conclusion presented dataset valuable resource for future research into this important molecular intersection heart.