Neofunctionalization of Chromoplast Specific Lycopene Beta Cyclase Gene (CYC-B) in Tomato Clade

• DOI: 10.1371/journal.pone.0153333
• Publication Date: 2016-04-12T17:33:44Z
• Authors: Vijee Mohan; Arun Pandey; Yellamaraju Sreel...; Rameshwar Sharma
• Keywords: Neofunctionalization

The ancestor of tomato underwent whole genome triplication ca. 71 Myr ago followed by widespread gene loss. However, few the triplicated genes are retained in modern day including lycopene beta cyclase that mediates conversion to β-carotene. fruit specific β-carotene formation is mediated a chromoplast-specific paralog (CYC-B) gene. Presently limited information available about how variations CYC-B contributed its neofunctionalization. clade contained several SNPs and In-Dels coding sequence (33 haplotypes) promoter region (44 haplotypes). appeared undergo purifying selection. transit peptide protein was predicted have stronger plastid targeting signal than chloroplast indicating possible In two Bog (Beta old gold) mutants, NUPT (nuclear plastid) DNA fragment 256 bp, likely derived from S. chilense accession, present. transient expression assay, this more efficient "Beta type" promoter. CARGATCONSENSUS box sequences required for binding MADS-box regulatory RIPENING INHIBITOR (RIN). loss may be related attenuation efficiency promote higher accumulation during ripening.