Profile of Class I Histone Deacetylases (HDAC) by Human Dendritic Cells after Alcohol Consumption and In Vitro Alcohol Treatment and Their Implication in Oxidative Stress: Role of HDAC Inhibitors Trichostatin A and Mocetinostat

• DOI: 10.1371/journal.pone.0156421
• Publication Date: 2016-06-02T00:59:06Z
• Authors: Marisela Agudelo; Gloria Figueroa; Tiyash Parira; Adriana Yndart; Karla Muñoz; Venkata Atluri; Thangavel Samikkannu; Madhavan P. Nair
• Keywords: Trichostatin A; Histone deacetylase inhibitor

Epigenetic mechanisms have been shown to play a role in alcohol use disorders (AUDs) and may prove be valuable therapeutic targets. However, the involvement of histone deacetylases (HDACs) on alcohol-induced oxidative stress human primary monocyte-derived dendritic cells (MDDCs) has not elucidated. In current study, we took novel approach combining ex vivo, vitro silico analyses elucidate HDACs periphery. vivo alcohol-modulation class I activity by MDDCs from self-reported users non-alcohol was performed. Additionally, treated with were assessed using qRT-PCR, western blot, fluorometric assay. The functional effects alcohol-induce measured PCR array gene expression network analysis. Our findings show, for first time, that higher levels compare controls treatment differentially modulates expression. Further, HDAC inhibitors (HDACi) blocked alcohol-induction modulated related genes expressed MDDCs. analysis revealed new target pathways mode action HDACi. Findings elucidating ability modulate useful damage delineate potential immune-modulatory mechanisms.