Identification and Validation of SNP Markers Linked to Dwarf Traits Using SLAF-Seq Technology in Lagerstroemia

• DOI: 10.1371/journal.pone.0158970
• Publication Date: 2016-07-12T20:18:29Z
• Authors: Yuanjun Ye; Ming Cai; Yiqian Ju; Yao Jiao; Lu Feng; Huitang Pan; Tangren Cheng; Qixiang Zhang
• Keywords: Marker-Assisted Selection; Molecular marker; Trait

The genetic control of plant architecture is a promising approach to breed desirable cultivars, particularly in ornamental flowers. In this study, the F1 population (142 seedlings) derived from Lagerstroemia fauriei (non-dwarf) × L. indica 'Pocomoke' (dwarf) was phenotyped for six traits (plant height (PH), internode length (IL), number, primary lateral branch (PLBH), secondary and number), IL PLBH were positively correlated with PH trait considered representative indexes PH. Fifty non-dwarf dwarf seedlings pooled subjected specific-locus amplified fragment sequencing (SLAF-seq) method, which screened 1221 polymorphic markers. A total 3 markers segregating between bulks validated population, M16337 M38412 highly M25207 marker trait. These provide predictability approximately 80% using single (M25207) 90% combinations (M16337 + M25207) revealed that traits, especially PLBH, decisive elements terms molecular regulation. Further validation performed BC1 set 28 stocks allele-specific PCR (AS-PCR) technology, results showed stability reliability SNP co-determination by multiple genes. Our findings an important theoretical practical basis early prediction indirect selection detected SNPs may be useful marker-assisted (MAS) crape myrtle.