Genetic Diversity and Phylogenetic Evolution of Tibetan Sheep Based on mtDNA D-Loop Sequences

• DOI: 10.1371/journal.pone.0159308
• Publication Date: 2016-07-27T17:41:07Z
• Authors: Jianbin Liu; Xuezhi Ding; Yufeng Zeng; Yaojing Yue; Xian Guo; Tingting Guo; Min Chu; Fan Wang; Jilong Han; Ruilin Feng; Xiaoping Sun; Chune Niu; Bohui Yang; Jian Guo; Chao Yuan
• Keywords: Haplogroup; Nucleotide diversity; D-loop; Phylogenetic diversity

The molecular and population genetic evidence of the phylogenetic status Tibetan sheep (Ovis aries) is not well understood, little known about this species' diversity. This knowledge gap partly due to difficulty sample collection. first work address question. Here, diversity relationship 636 individual from fifteen populations were assessed using 642 complete sequences mitochondrial DNA D-loop. Samples collected Qinghai-Tibetan Plateau area in China, reference data obtained six breed available GenBank. length varied considerably, between 1031 1259 bp. haplotype nucleotide 0.992±0.010 0.019±0.001, respectively. average number differences was 19.635. mean composition 350 haplotypes 32.961% A, 29.708% T, 22.892% C, 14.439% G, 62.669% A+T, 37.331% G+C. Phylogenetic analysis showed that all four previously defined haplogroups (A, B, D) found individuals but only D haplogroup Linzhou sheep. Further, clustering divided into at least two clusters. estimation demographic parameters mismatch analyses C had one expansion These results contribute will help inform future conservation programs native Plateau.