New Biochemical Insights into the Mechanisms of Pulmonary Arterial Hypertension in Humans

• DOI: 10.1371/journal.pone.0160505
• Publication Date: 2016-08-03T17:33:19Z
• Authors: Renata Bujak; Jesús Mateo; Isabel Blanco; José Luis Izquier...; Danuta Dudzik; Michał J. Markusz...; Victor Ivo Peinado; Martín Laclaustra; Joan Albert Barberá; Coral Barbas; Jesús Ruiz-Cabello
• Keywords: Pathophysiology of hypertension

Diagnosis of pulmonary arterial hypertension (PAH) is difficult due to the lack specific clinical symptoms and biomarkers, especially at early stages. We compared plasma metabolic fingerprints PAH patients (n = 20) with matched healthy volunteers using, for first time, untargeted multiplatform metabolomics approach consisting high-performance liquid gas chromatography coupled mass spectrometry. Multivariate statistical analyses were performed select metabolites that contribute most groups' classification (21 from in both ionization modes 9 chromatography-mass spectrometry). found related energy imbalance, such as glycolysis-derived metabolites, well involved fatty acid, lipid amino acid metabolism. observed statistically significant changes threitol aminomalonic patients, which could provide new biochemical insights into pathogenesis disease. The results externally validated on independent case control cohorts, confirming up 16 validation study. Multiplatform metabolomics, followed by multivariate chemometric data analysis has a huge potential explaining searching more less invasive markers