Volatile Organic Compound Gamma-Butyrolactone Released upon Herpes Simplex Virus Type -1 Acute Infection Modulated Membrane Potential and Repressed Viral Infection in Human Neuron-Like Cells

• DOI: 10.1371/journal.pone.0161119
• Publication Date: 2016-08-18T17:51:25Z
• Authors: Kevin Rochford; Feng Chen; Yan Waguespack; Robert W. Figliozzi; Madan K. Kharel; Qiaojuan Zhang; Miguel Martin-Car...; S. Victor Hsia
• Keywords: Vero cell

Herpes Simplex Virus Type -1 (HSV-1) infections can cause serious complications such as keratitis and encephalitis. The goal of this study was to identify any changes in the concentrations volatile organic compounds (VOCs) produced during HSV-1 infection epithelial cells that could potentially be used an indicator a response stress. An additional objective if VOCs released from acute may influence subsequent neuronal facilitate latency. To investigate these hypotheses, Vero were infected with emission analyzed using two-dimensional gas chromatograph/mass spectrometry (2D GC/MS). It observed gamma-butyrolactone (GBL) particular changed significantly after 24-hour infection. Since establish latency neurons infection, GBL tested determine it exerts regulation results indicated altered resting membrane potential differentiated LNCaP promoted non-permissive state by repressing viral replication. These observations provide useful clues towards understanding complex signaling pathways occur primary establishment