Dose-Related and Time-Dependent Development of Collagenase-Induced Tendinopathy in Rats

• DOI: 10.1371/journal.pone.0161590
• Publication Date: 2016-08-22T13:41:10Z
• Authors: Carlotta Perucca ...; Arianna B. Lovati; Marco Viganò; Deborah Stanco; Marta Bottagisio; Alessia Di Gianca...; Stefania Setti; Laura de Girolamo
• Keywords: Male; Time Factors; Science; Achilles Tendon; Injections; Rats, Sprague-Dawley; 03 medical and health sciences; 0302 clinical medicine; Animals; Humans; Agricultural and Biological Sciences (all); Biochemistry, Genetics and Molecular Biology (all); Medicine (all); Collagenases; Wound Healing; Dose-Response Relationship, Drug; Neovascularization, Pathologic; Q; R; Tendinopathy, animal model, collagenase; Rats; 3. Good health; Disease Models, Animal; Tendinopathy; Disease Progression; Medicine; Collagen; Research Article

Tendinopathy is a big burden in clinics and it represents 45% of musculoskeletal lesions. Despite the relevant social impact, both pathogenesis development tendinopathy are still under-investigated, thus limiting therapeutic advancement this field. The purpose study was to evaluate dose-dependent time-related tissue-level changes occurring collagenase-induced rat Achilles tendons, order establish standardized model for future pre-clinical studies. With purpose, 40 Sprague Dawley rats were randomly divided into two groups, treated by injecting collagenase type I within tendon at 1 mg/mL (low dose) or 3 (high dose). Tendon explants histologically evaluated 3, 7, 15, 30 45 days. Our results revealed that doses induced disorganization collagen fibers increased number rounded resident cells. In particular, high dose treatment determined greater neovascularization fatty degeneration with respect lower dose. These found be time-dependent resemble features human tendinopathy. Indeed, our series, acute phase occurred from day then progressed towards proliferative displaying degenerative appearance associated very precocious mild remodeling process. good balance between similarity histological feasibility, terms size create lesions costs when compared other animal models. Moreover, could contribute improve knowledge field, useful properly design further studies test innovative treatments