Differences in Circulating microRNAs between Grazing and Grain-Fed Wagyu Cattle Are Associated with Altered Expression of Intramuscular microRNA, the Potential Target PTEN, and Lipogenic Genes

• DOI: 10.1371/journal.pone.0162496
• Publication Date: 2016-09-09T14:26:30Z
• Authors: Susumu Muroya; Masahiro Shibata; Masayuki Hayashi; Mika Oe; Koichi Ojima
• Keywords: Beef Cattle

We aimed to understand the roles of miRNAs in muscle tissue maturation and those circulating microRNAs (c-miRNAs) beef production Japanese Black (JB) cattle (Wagyu), a breed with genetically background superior intermuscular fat depot, by comparing different feeding conditions (indoor grain-feeding vs. grazing on pasture). The at 18 months old were assigned pasture or conventional indoor grain for 5 months. Microarray analysis c-miRNAs from plasma extracellular vesicles led detection total 202 bovine plasma, including 15 that differed between conditions. Validation microarray results qPCR showed miR-10b level was upregulated compared grain-fed cattle. In contrast, levels miR-17-5p, miR-19b, miR-29b, miR-30b-5p, miR-98, miR-142-5p, miR-301a, miR-374b, miR-425-5p, miR-652 lower than Bioinformatic indicated predicted target genes enriched gene ontology terms associated blood vessel morphogenesis, membrane, focal adhesion, endocytosis, collagen, ECM-receptor interaction, phosphorylation. cattle, elevation expression coincident its longissimus lumborum (LL) muscle. Expression bovine-specific miR-2478, most plasma-enriched miRNA, tended be also but not plasma. Furthermore, caused downregulated mRNA and/or miR-2478 genes, such as DNAJB2, PTEN, SCD1. Thus, system used JB affected could indicators feeding. Among these, especially feeding-induced changes potential responsible glucose homeostasis intramuscular depot LL