Development and Evaluation of a Novel Mucoadhesive Film Containing Acmella oleracea Extract for Oral Mucosa Topical Anesthesia

• DOI: 10.1371/journal.pone.0162850
• Publication Date: 2016-09-14T13:45:56Z
• Authors: Verônica Santana ...; Michelle Franz-Mo...; Francisco Carlos ...; João Ernesto de C...; Glyn Mara Figueira; Luciano Serpe; Ilza Maria Olivei...; Viviane Aparecida...; Lais Thiemi Yamane; Eneida de Paula; Rodney Alexandre ...
• Keywords: Maceration (sewage); Topical anesthetic; Oral mucosa

To develop an anesthetic mucoadhesive film containing Acmella oleracea (jambu) extract for topical use on oral mucosa.Ethanolic extracts from aerial parts of jambu were prepared by maceration. Pigment removal was obtained adsorption with activated carbon. Three films developed using a casting method: 10 or 20% crude (10% JB and JB), 10% treated carbon JBC). The characterized regarding their uniformity, thickness, pH, spilanthol content, stability evaluated during 120 days. Gas chromatography used to quantify the amount spilanthol. In vitro tests determined permeation across pig esophageal epithelium mucosa in Franz diffusion cells. Topical efficacy assessed vivo tail flick test mice.The three showed physical visual appearances suitable mucosa. study revealed that JBC presented higher flux permeability coefficient values, compared (p < 0.001). Moreover, better than other 0.01).Mucoadhesive is potential alternative oral, use, encouraging future clinical studies.