Transcriptome Dynamics during Maize Endosperm Development

• DOI: 10.1371/journal.pone.0163814
• Publication Date: 2016-10-03T18:04:50Z
• Authors: Jianzhou Qu; Chuang Ma; Jiaojiao Feng; Shutu Xu; Lei Wang; Feifei Li; Yibo Li; Renhe Zhang; Xinghua Zhang; Jiquan Xue; Dongwei Guo
• Keywords: WRKY protein domain

The endosperm is a major organ of the seed that plays vital roles in determining weight and quality. However, genome-wide transcriptome patterns throughout maize development have not been comprehensively investigated to date. Accordingly, we performed high-throughput RNA sequencing (RNA-seq) analysis at 5, 10, 15 20 days after pollination (DAP). We found more than 11,000 protein-coding genes underwent alternative splicing (AS) events during four developmental stages studied. These were mainly involved intracellular protein transport, signal transmission, cellular carbohydrate metabolism, lipid biosynthesis, modification, histone amino acid DNA repair. Additionally, 7,633 genes, including 473 transcription factors (TFs), differentially expressed among stages. TFs from 50 families, bZIP, WRKY, GeBP ARF families. Further stage-specific showed binding, nucleus ligand-dependent nuclear receptor activities might be important 5 DAP, immune responses, signalling, binding lumen are 10 metabolic processes cytoplasm responses various stimuli different DAP compared with other This RNA-seq provides novel, comprehensive insights into dynamics early maize.