Immunological Signatures after Bordetella pertussis Infection Demonstrate Importance of Pulmonary Innate Immune Cells
CD4-Positive T-Lymphocytes
Whooping Cough
Science
Q
R
Antibodies, Bacterial
Bordetella pertussis
Immunity, Innate
3. Good health
Mice
03 medical and health sciences
0302 clinical medicine
Medicine
Animals
Cytokines
Lung
Spleen
Research Article
DOI:
10.1371/journal.pone.0164027
Publication Date:
2016-10-06T17:38:04Z
AUTHORS (10)
ABSTRACT
Effective immunity against Bordetella pertussis is currently under discussion following the stacking evidence of resurgence in vaccinated population. Natural more effective than vaccine-induced indicating that knowledge on infection-induced responses may contribute to improve vaccination strategies. We applied a systems biology approach comprising microarray, flow cytometry and multiplex immunoassays unravel molecular cellular signatures unprotected mice protected with immunity, around B. challenge. Pre-existing systemic memory Th1/Th17 cells, B-cells, mucosal IgA specific for Ptx, Vag8, Fim2/3 were detected 56 days after an experimental infection. In addition, pre-existing high activity reactivation pulmonary innate cells such as alveolar macrophages, M-cells goblet was detected. The pro-inflammatory lungs serum, neutrophil recruitment spleen upon infectious challenge absent mice. Instead, fast immune led efficient bacterial clearance harbored potential new gene markers pertussis. These comprised makers, Clca3, Retlna, Glycam1, Gp2, Umod, next adaptive markers, CCR6+ Th17 CXCR6+ T-cells demonstrated by transcriptome analysis. conclusion, besides responses, primary benefits from activation resident achieved local pathogen-recognition. provided
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