Anti-Arthritic Activity of Schistosoma mansoni and Trichinella spiralis Derived-Antigens in Adjuvant Arthritis in Rats: Role of FOXP3+ Treg Cells

• DOI: 10.1371/journal.pone.0165916
• Publication Date: 2016-11-01T17:31:48Z
• Authors: Maha M. Eissa; Dalia K. Mostafa; Amany A. Ghazy; Mervat Z. El azzouni; Laila M. Boulos; Layla K. Younis
• Keywords: Intradermal injection

A growing body of evidence supports the concept helminths therapy in a variety autoimmune diseases. Here, we aimed to investigate protective effects autoclaved Schistosoma mansoni antigen (ASMA) and Trichinella spiralis (ATSA) on clinical immunopathological features rheumatoid arthritis (RA). Adjuvant was induced by subcutaneous intradermal injections complete Freund’s adjuvant into plantar surface right hind paw root tail, respectively. Rats were randomly assigned serve as normal control, untreated arthritis, ASMA or ATSA-treated groups. Antigens given injection two doses, weeks apart. The development, progression arthritic features, impact animals’ gait weight followed up for 4 weeks. associated changes serum cytokines (IL-17, IFN-γ IL-10), joints’ histopathology immunohistochemistry Foxp3+ T regulatory cells (Tregs) evaluated at end study. Treatment with either ATSA attenuated polyarthritis, improved gain, reduced elevated IL-17 further increased both IL-10. Histopathologically, this remarkable regression paws’ inflammation that limited only tissue, significant increase number Foxp 3+ versus group. In conclusion, derived antigens exerted effect against better achieved treatment. This anti-arthritic activity is attributed upregulation Tregs, subsequent favorable modulation pro- anti-inflammatory cytokines. use parasitic excludes deleterious imposing helminthic infection using live parasites, which may pave way new therapeutic modality treating RA.