The Pig: A Relevant Model for Evaluating the Neutrophil Serine Protease Activities during Acute Pseudomonas aeruginosa Lung Infection

• DOI: 10.1371/journal.pone.0168577
• Publication Date: 2016-12-19T13:44:19Z
• Authors: Claire Chevaleyre; Mickaël Riou; Déborah Bréa; Clarisse Vandebrouck; Céline Barc; Jérémy Pezant; Sandrine Melo; Michel Olivier; Rémy Delaunay; Olivier Boulesteix; Patricia Berthon; Christelle Rossignol; Julien Burlaud Ga...; Frédéric Becq; Francis Gauthier; Mustapha Si-Tahar; François Meurens; Mustapha Berri; Ignacio Caballero...; Sylvie Attucci
• Keywords: Proinflammatory cytokine

The main features of lung infection and inflammation are a massive recruitment neutrophils the subsequent release neutrophil serine proteases (NSPs). Anti-infectious and/or anti-inflammatory treatments must be tested on suitable animal model. Mice models do not replicate several aspects human disease. This is particularly true for cystic fibrosis (CF), which has led scientific community to search new models. We have shown that mice appropriate characterizing drugs targeting neutrophil-dependent pig their NSPs similar homologues. induced acute neutrophilic inflammatory responses in lungs using Pseudomonas aeruginosa, an opportunistic respiratory pathogen. Blood samples, nasal swabs bronchoalveolar lavage fluids (BALFs) were collected at 0, 3, 6 24 h post-insfection (p.i.) biochemical parameters, serum BAL cytokines, bacterial cultures activity evaluated. proinflammatory mediators, hematological blood cell bronchial reactivity, peaked 6h p.i.. also used synthetic substrates specific show BALFs increased. These detected surface anti-human NSP antibodies. aeruginosa-induced pigs results response described ventilator-associated pneumonia humans. Altogether, this indicates model testing anti-infectious combat adverse proteolytic effects diseases.