Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering

• DOI: 10.1371/journal.pone.0171231
• Publication Date: 2017-01-31T18:49:29Z
• Authors: Clara Sanjurjo-Ro...; Rocío Castro-Viñu...; Tamara Hermida-Gómez; Tania Fernández-V...; Isaac Manuel Fuen...; Francisco Javier ...; Silvia María Díaz...; Francisco Javier ...
• Keywords: Fibrocartilage; Hyaline cartilage

Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment osteochondral diseases.The aim was to isolate and characterize from bone marrow aspirates (oBMSCs) assess their usefulness repair using β-tricalcium phosphate (bTCP) type I collagen (Col I) scaffolds.Cells isolated were characterized morphologically, phenotypically, functionally. oBMSCs cultured osteogenic medium on bTCP Col scaffolds. The resulting constructs evaluated by histology, immunohistochemistry electron microscopy studies. Furthermore, scaffolds develop an in vitro cartilage model that assessed a modified International Cartilage Research Society (ICRS) II scale.oBMSCs presented morphology, surface marker pattern multipotent capacities similar those human BMSCs. seeded gave rise neotissue. Assessment ICRS scale revealed fibrocartilage/hyaline obtained model.The demonstrated be oBMSCs. sponges successfully synthesized tissue. data suggest have potential use preclinical models prior clinical