Functional MRI for characterization of renal perfusion impairment and edema formation due to acute kidney injury in different mouse strains

• DOI: 10.1371/journal.pone.0173248
• Publication Date: 2017-03-20T17:26:57Z
• Authors: Susanne Tewes; Faikah Gueler; Rongjun Chen; Marcel Gutberlet; Mi-Sun Jang; Martin Meier; Michael Mengel; Dagmar Hartung; Frank Wacker; Song Rong; Katja Hueper
• Keywords: Renal ischemia

Purpose The purpose was to characterize acute kidney injury (AKI) in C57BL/6 (B6)- and 129/Sv (Sv)-mice by noninvasive measurement of renal perfusion tissue edema using functional MRI. Methods Different severities AKI were induced B6- Sv-mice ischemia reperfusion (IRI). Unilateral clamping the pedicle for 35 min (moderate AKI) or 45 (severe done. MRI (7-Tesla) performed 1, 7 28 days after surgery a flow alternating inversion recovery (FAIR) arterial spin labeling (ASL) sequence. Maps T1-relaxation time calculated. Relative MRI-parameters IRI compared contralateral not-clipped between mouse strains unpaired t-tests. In addition, fibrosis assessed Masson Trichrome collagen IV staining. Results After moderate relative impairment significantly higher than at d7 (55±7% vs. 82±8%, p<0.05) d28 (76±7% 102±3%, p<0.01). T1-values increased early phase both strains. T1-increase more severe prolonged times strains, measured cortex outer stripe medulla. Kidney volume loss (compared kidney) occurred already but proceeded markedly towards 4 weeks AKI. Early predictive later loss. progression chronic disease (CKD) model similar as revealed histology. Conclusion Quantification allows characterization strain differences upon Renal stronger Sv-animals following Prolonged associated with formation CKD within observation.