Comparative analysis of the expression level of recombinant ginsenoside-transforming β-glucosidase in GRAS hosts and mass production of the ginsenoside Rh2-Mix

• DOI: 10.1371/journal.pone.0176098
• Publication Date: 2017-04-19T17:59:34Z
• Authors: Muhammad Zubair S...; Chang-Hao Cui; Seul-Ki Park; Nam Soo Han; Sun-Chang Kim; Wan-Taek Im
• Keywords: Biotransformation; Bioconversion

The ginsenoside Rh2, a pharmaceutically active component of ginseng, is known to have anticancer and antitumor effects. However, white ginseng red extremely low concentrations Rh2 or Rh2-Mix [20(S)-Rh2, 20(R)-Rh2, Rk2, Rh3]. To enhance the production food-grade an edible enzymatic bioconversion technique was developed adopting GRAS host strains. A β-glucosidase (BglPm), which has conversion ability, expressed in three strains (Corynebacterium glutamicum, Saccharomyces cerevisiae Lactococus lactis) by using different vector system. Enzyme activity these hosts were 75.4%, 11.5%, 9.3%, respectively, compared that E. coli pGEX 4T-1 expression highly BglPm_C C. glutamicum can effectively transform Rg3-Mix [20(S)-Rg3, 20(R)-Rg3, Rk1, Rg5] Rh3] scaled-up biotransformation reaction, performed 10-L jar fermenter at pH 6.5/7.0 37°C for 24 h. our knowledge, this first report 50 g PPD-Mix (Rb1, Rb2, Rb3, Rc, Rd) as starting substrate converted acid heat treatment then 24.5-g obtained transformation through BglPm_C. Utilization method could be usefully exploited preparation cosmetics, functional food, pharmaceutical industries, thereby replacing