Effect of chronic estradiol plus progesterone treatment on experimental arterial and venous thrombosis in mouse

• DOI: 10.1371/journal.pone.0177043
• Publication Date: 2017-05-09T19:22:49Z
• Authors: Marie-Cécile Valéra; Emmanuelle Noirri...; Marion Dupuis; Melissa Buscato; Alexia Vinel; Maeva Guillaume; Anne Briaux; Cédric Garcia; Thibaut Benoit; Olivier Lairez; Coralie Fontaine; Bernard Payrastre; Jean-François Arnal
• Keywords: Progestin; Progestogen

Postmenopausal hormone replacement therapy (HRT) with estrogen plus progestogens is the first line to treat menopausal symptoms. The progestogen added reduce or eliminate excess risk of endometrial cancer due unopposed effect estrogen. Whereas progestin clearly opposes proliferative and deleterious long-term actions on endometrium, interference other action remains unclear. We previously reported that chronic subcutaneous 17α-estradiol (E2) in mice decreases platelet responsiveness, prolongs tail-bleeding time protects against acute thromboembolism. Here, we report tissue-specific progesterone (P4) E2 ovariectomized mice. confirm that, our experimental conditions, P4 attenuates uterus effects vagina weight lubrication. then studied combined hemostasis thrombosis vivo found did not interfere main platelets, bleeding arterial venous thrombosis. Thus, whereas activation receptor interferes its classic sex targets, appears have minimal E2, supporting prominent role estrogens accessory natural extra-reproductive cells tissues involved