Different adaptation strategies of two citrus scion/rootstock combinations in response to drought stress

Citrus http://aims.fao.org/aos/agrovoc/c_1647 F60 - Physiologie et biochimie végétale Science http://aims.fao.org/aos/agrovoc/c_27639 Plant Roots F30 - Génétique et amélioration des plantes http://aims.fao.org/aos/agrovoc/c_6653 Polyploidy 03 medical and health sciences http://aims.fao.org/aos/agrovoc/c_2392 Gene Expression Regulation, Plant http://aims.fao.org/aos/agrovoc/c_6857 Protein Interaction Maps métabolisme Plant Proteins 2. Zero hunger 0303 health sciences http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_1637 Gene Expression Profiling Q http://aims.fao.org/aos/agrovoc/c_4769 R greffon 15. Life on land adaptation physiologique http://aims.fao.org/aos/agrovoc/c_27527 Adaptation, Physiological 6. Clean water résistance à la sécheresse Droughts porte greffe http://aims.fao.org/aos/agrovoc/c_1070 Medicine H50 - Troubles divers des plantes Citrus sinensis expression des gènes stress dû à la sécheresse Research Article
DOI: 10.1371/journal.pone.0177993 Publication Date: 2017-05-18T19:17:02Z
ABSTRACT
Scion/rootstock interaction is important for plant development and for breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. Here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) rootstocks grafted with 2x Valencia Delta sweet orange (Citrus sinensis) scions, named V/2xRL and V/4xRL, respectively. Based on previous gene expression data, we developed an interactomic approach to identify proteins involved in V/2xRL and V/4xRL response to drought. A main interactomic network containing 3,830 nodes and 97,652 edges was built from V/2xRL and V/4xRL data. Exclusive proteins of the V/2xRL and V/4xRL networks (2,056 and 1,001, respectively), as well as common to both networks (773) were identified. Functional clusters were obtained and two models of drought stress response for the V/2xRL and V/4xRL genotypes were designed. Even if the V/2xRL plant implement some tolerance mechanisms, the global plant response to drought was rapid and quickly exhaustive resulting in a general tendency to dehydration avoidance, which presented some advantage in short and strong drought stress conditions, but which, in long terms, does not allow the plant survival. At the contrary, the V/4xRL plants presented a response which strong impacts on development but that present some advantages in case of prolonged drought. Finally, some specific proteins, which presented high centrality on interactomic analysis were identified as good candidates for subsequent functional analysis of citrus genes related to drought response, as well as be good markers of one or another physiological mechanism implemented by the plants.
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