Functional interaction between co-expressed MAGE-A proteins

• DOI: 10.1371/journal.pone.0178370
• Publication Date: 2017-05-24T17:44:48Z
• Authors: Julieta E. Laiseca; María F. Ladelfa; Javier Cotignola; Leticia Y. Peche; Franco A. Pascucci; Bryan A. Castaño; Mario D. Galigniana; Claudio Schneider; Martin Monte
• Keywords: Male; 0301 basic medicine; Science; Gene Expression; 03 medical and health sciences; Testicular Neoplasms; Antigens, Neoplasm; https://purl.org/becyt/ford/1.6; Cell Line, Tumor; Humans; Protein Interaction Domains and Motifs; https://purl.org/becyt/ford/1; Ar; Protein Stability; Q; R; Ubiquitination; Prostatic Neoplasms; Neoplasms, Germ Cell and Embryonal; Mage; Antigens, Neoplasm; Cell Line, Tumor; Gene Expression; Humans; Male; Multiprotein Complexes; Neoplasm Proteins; Neoplasms, Germ Cell and Embryonal; Prostatic Neoplasms; Protein Interaction Domains and Motifs; Protein Stability; Receptors, Androgen; Testicular Neoplasms; Ubiquitination; Medicine (all); Biochemistry, Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all); Neoplasm Proteins; 3. Good health; Receptors, Androgen; Multiprotein Complexes; Medicine; Stability; Research Article

MAGE-A (Melanoma Antigen Genes-A) are tumor-associated proteins with expression in a broad spectrum of human tumors and normal germ cells. gene function being increasingly investigated to better understand the mechanisms by which MAGE collaborate tumorigenesis whether their detection could be useful for disease prognosis purposes. Alterations epigenetic involved silencing cause frequent co-expression tumor Here, we have analyzed effect our results suggest that MageA6 can potentiate androgen receptor (AR) co-activation MageA11. Database search confirmed MageA11 co-expressed prostate cancer samples. We demonstrate form protein complex resulting stabilization consequently enhancement AR activity. The mechanism involves association Mage A6-MHD domain MageA11, prevention ubiquitinylation on lysines 240 245 decreased proteasome-dependent degradation. experimentally here first time two act together non-redundant way specific oncogenic function. Overall, highlight complexity networking regulating cell behavior.