Actinic keratosis modelling in mice: A translational study

• DOI: 10.1371/journal.pone.0179991
• Publication Date: 2017-06-29T17:46:05Z
• Authors: Arnaud Pillon; Bruno Gomes; Isabelle Vandenbe...; Valérie Cartron; Patrick Cèbe; Jean-Christophe B...; Vincent Sibaud; Nicolas Guilbaud; Laurent Audoly; Laurence Lamant; Anna Kruczynski
• Keywords: Actinic keratosis; Acanthosis; Human skin; Histology; Seborrheic keratosis

Background Actinic keratoses (AK) are pre-malignant cutaneous lesions caused by prolonged exposure to ultraviolet radiation. As AKs generally accepted be the initial in a disease continuum that progresses squamous cell carcinoma (SCC), AK have treated. They also second most common reason for visits dermatologist. Several treatments available but their efficacy still needs improved. The UV-B-induced KA lesion mouse model is used preclinical studies assess of novel molecules, even though it often more representative advanced or SCC. Objectives Here we report on translational study, comparing various stages development humans and UV-B irradiated model, as well optimization photograph acquisition skin. Methods Human skin were analysed histology immunohistochemistry. Mouse assessed using digital dermatoscope. Results An histological phenotypic analysis, including p53, Ki67 CD3 expression detection, performed human lesions, shows overall modelling mice relevant clinical situation. Some differences observed, such disorganization keratinocytes basal layer number atypical nuclei which numerous AK, whereas much pronounced acanthosis observed mice. Thanks this able select appropriate experimental conditions establishing either early stage an SCC model. Furthermore, optimized dermatoscope clinics allows reproducible further reliable assessment lesions. Use camera illustrated through pharmacological study assessing activity CARAC®. Conclusion These data demonstrate predictive identification therapeutic both disease.