Nutritional and reproductive signaling revealed by comparative gene expression analysis in Chrysopa pallens (Rambur) at different nutritional statuses

• DOI: 10.1371/journal.pone.0180373
• Publication Date: 2017-07-06T17:38:58Z
• Authors: Benfeng Han; Shen Zhang; Fanrong Zeng; Jianjun Mao
• Keywords: Vitellogenins; KEGG; Vitellogenin; Illumina dye sequencing

The green lacewing, Chrysopa pallens Rambur, is one of the most important natural predators because its extensive spectrum prey and wide distribution. However, what we know about nutritional reproductive physiology this species very scarce.By cDNA amplification Illumina short-read sequencing, analyzed transcriptomes C. female adult under starved fed conditions. In total, 71236 unigenes were obtained with an average length 833 bp. Four vitellogenins, three insulin-like peptides two insulin receptors annotated. Comparison gene expression profiles suggested that totally 1501 genes differentially expressed between statuses. KEGG orthology classification showed these (DEGs) mapped to 241 pathways. turn, top 4 are ribosome, protein processing in endoplasmic reticulum, biosynthesis amino acids carbon metabolism, indicating a distinct difference signaling feeding conditions.Our study yielded large-scale molecular information relevant signaling, which will contribute mass rearing commercial use predaceous insect species.