Plasma metabolomics profiles in rats with acute traumatic brain injury

• DOI: 10.1371/journal.pone.0182025
• Publication Date: 2017-08-03T17:44:10Z
• Authors: Fei Zheng; Zi-An Xia; Yi-Fu Zeng; Jie-Kun Luo; Peng Sun; Han-Jin Cui; Yang Wang; Tao Tang; Yan-Tao Zhou
• Keywords: Metabolome

Traumatic brain injury (TBI) is a major cause of mortality and disability worldwide. We validated the utility plasma metabolomics analysis in clinical diagnosis acute TBI rat model controlled cortical impact (CCI) using gas chromatography/mass spectrometry (GC/MS). Thirty Sprague-Dawley rats were randomly divided into two groups 15 each: CCI group sham group. Blood samples obtained from within first 24 h after injury. GC/MS measurements performed to evaluate profile TBI-induced metabolic changes, resulting identification 45 metabolites plasma. Principal component analysis, partial least squares-discriminant orthogonal square discriminant hierarchical clustering univariate/multivariate analyses revealed clear differences metabolome between induced distinctive changes including linoleic acid metabolism, amino galactose arachidonic metabolism. Specifically, exhibited significant alterations proline, phosphoric acid, β-hydroxybutyric galactose, creatinine, L-valine, acid. A receiver operating characteristic curve showed that above 8 could be used as potential biomarkers for TBI. Furthermore, this study time identify biomarker candidate This comprehensive complements target screening diagnostic enhances predictive value therapeutic intervention