Changes in the pattern of plasma extracellular vesicles after severe trauma

• DOI: 10.1371/journal.pone.0183640
• Publication Date: 2017-08-24T17:43:59Z
• Authors: Sahithi J. Kuravi; Clara M. Yates; Mark Foster; Paul Harrison; Jon Hazeldine; Peter Hampson; Chris Watson; Antonio Belli; Mark Midwinter; Gerard B. Nash
• Keywords: Nanoparticle tracking analysis; Extracellular Vesicles; Blood plasma

Extracellular vesicles (EV) released into the circulation after traumatic injury may influence complications. We thus evaluated numbers of EV in plasma over 28 days trauma and their pro-coagulant inflammatory effects.37 patients suffering with an severity score >15 were studied along 24 healthy controls. Plasma samples isolated by double centrifugation (2000g 20min; 13000g 2min) from blood collected within hour up to injury. counted sized using nanoparticle tracking analysis (NTA); counts cellular origins also determined flow cytometry (FC) cell-specific markers. Functional effects tested a procoagulant phospholipid assay flow-based, leukocyte adhesion endothelial cells (EC) treated EV. found that concentrations measured NTA significantly increased compared controls, remained elevated days. In addition, or FC showed had higher derived platelets (CD41+), leukocytes (CD45+) EC (CD144+). The increases evident throughout 28-day follow-up. However, count represented <1% detected NTA, only 1-2% identified diameter >400nm. activity patient accelerated coagulation on day 1 3 trauma, times correlated counts. Furthermore, treatment for hours containing tended increase recruitment peripheral flowing mononuclear cells.EV represent small sub-population total load NTA. Both methods however indicate significant severe have pro-inflammatory outcomes.