The microbiota of water buffalo milk during mastitis

• DOI: 10.1371/journal.pone.0184710
• Publication Date: 2017-09-19T13:31:50Z
• Authors: Carlotta Catozzi; Armand Sanchez Bo...; Olga Francino; Cristina Lecchi; Esterina De Carlo; Domenico Vecchio; Alessandra Martuc...; Pasquale Fraulo; Valerio Bronzo; Anna Cuscó; Sara D’Andreano; Fabrizio Ceciliani
• Keywords: Udder; Somatic Cell Count; Propionibacterium; Micrococcus

The aim of this study was to define the microbiota water buffalo milk during sub-clinical and clinical mastitis, as compared healthy status, by using high-throughput sequencing 16S rRNA gene. A total 137 quarter samples were included in experimental design: 27 derived from healthy, culture negative quarters, with a Somatic Cell Count (SCC) less than 200,000 cells/ml; quarters mastitis; 83 collected subclinical SCC number greater cells/ml and/or positive for udder pathogens, without signs mastitis. Bacterial DNA purified genes individually amplified sequenced. Significant differences found those diversity richer whose turn core milk, defined asset microorganisms shared all samples, includes 15 genera, namely Micrococcus, Propionibacterium, 5-7N15, Solibacillus, Staphylococcus, Aerococcus, Facklamia, Trichococcus, Turicibacter, 02d06, SMB53, Clostridium, Acinetobacter, Psychrobacter Pseudomonas. Only two genera (Acinetobacter Pseudomonas) present no genus across mastitis samples. presence be related change relative abundance such Psychrobacter, decreased 16.26% 3.2% Other SMB53 well. Discriminant analysis presents evidence that microbial community could discriminated on background their profiles.