Acute toxicity and genotoxicity of silver nanoparticle in rats

• DOI: 10.1371/journal.pone.0185554
• Publication Date: 2017-09-27T17:45:53Z
• Authors: Hairuo Wen; Mo Dan; Ying Yang; Jianjun Lyu; Anliang Shao; Xiang Cheng; Liang Chen; Liming Xu
• Keywords: Silver nanoparticle

The potential risk of a nanoparticle as medical application has raised wide concerns, and this study aims to investigate silver (AgNP)-induced acute toxicities, genotoxicities, target organs the underlying mechanisms.Sprague-Dawley rats were randomly divided into 4 groups (n = each group), AgNP (containing Ag nanoparticles released Ag+, 5 mg/kg), Ag+ (released from same dose AgNP, 0.0003 5% sucrose solution (vechicle control) cyclophophamide (positive control, 40 mg/kg) administrated intravenously for 24 h respectively. Clinical signs body weight recorded, tissues subsequently collected biochemical examination, distribution detection, histopathological examination genotoxicity assays.The rank detected in highest lowest is lung>spleen>liver>kidney>thymus>heart. Administration induced marked increase ALT, BUN, TBil Cre. Histopathological results showed that more extensive organ damages liver, kidneys, thymus, spleen. Bone marrow micronucleus assay found no statistical significance among (p > 0.05), but number aberration cells multiple predominately increased dosed with < 0.01), polyploidy generated group (4.3%) compared control.Our indicated accumulated immune system organs, mild irritation was observed thymus spleen animals treated not Ag+. liver kidneys could be most affected by an i.v. significantly chromosome breakage cell rates also implied AgNP. However, particle-specific toxicities carcinogenic effect remain further confirmed chronic toxicity study.