Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing

• DOI: 10.1371/journal.pone.0186051
• Publication Date: 2017-10-23T17:58:15Z
• Authors: Polina Galitskaya; Liliya Biktasheva; Anatoly Saveliev; Tatiana Grigoryeva; Eugenia Boulygina; Svetlana Selivano...
• Keywords: Pyrosequencing; Decomposer; Sawdust; Green waste; Sewage sludge

Composting is viewed as one of the primary methods to treat organic wastes. Co-composting may improve efficiency this treatment by establishing most suitable conditions for decomposers than those present in individual Given that bacteria and fungi are driving agents composting, information about composition their communities dynamics during composting reproducibility, performance quality final compost well help evaluate potential human health risk choice appropriate application procedure. In study, co-composting mixtures containing two similar components (organic fraction municipal solid waste sawdust polluted oil) discriminate component (sewage sludges different origin) were investigated. Bacterial fungal community successions analyzed process determining change structural using qPCR 454 pyrosequencing a lab experiment period 270 days. During initial stage, number 16S bacterial copies was (3.0±0.2) x 106 (0.4±0.0) 107 g-1, Rhodospiralles Lactobacialles orders dominated. Fungal had (2.9±0.0) x105 (6.1±0.2) ITS Saccharomycetales order At end thermophilic stage on 30th day underwent significant changes: dominants changed relative abundance decreased. Typical residents included Flavobacteriales, Chitinophagaceae Bacterioidetes Microascaceae, Dothideomycetes, Eurotiomycetes, Sordariomycetes, Agaricomycetes fungi. later stages, dominating taxa both remained, while accordance with OTUs, it concluded not similar. Analysis non-metric multidimensional scaling (NMDS) revealed composts became progressively more similar; trend followed community.